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SIFT: predicting amino acid changes that affect protein function
Solitary nucleotide polymorphism (SNP) studies and random mutagenesis tasks identify amino acid substitutions in protein-coding areas. Each replacement has got the prospective to impact protein function. SIFT ( S orting we ntolerant F rom T olerant) is really a system that predicts whether an acid that is amino impacts protein function to ensure that users can focus on substitutions for further research. We now have shown that SIFT can differentiate between functionally basic and deleterious amino acid alterations in mutagenesis studies as well as on human being polymorphisms. SIFT can be obtained at http://blocks.fhcrc.org/sift/SIFT.html.
Received January 4, 2003; Revised and February that is accepted 28 2003
Solitary nucleotide polymorphisms (SNPs) are employed as markers in linkage and relationship studies to identify which regions into the human being genome may be concerned in infection. SNPs in coding and regulatory areas may be implicated in infection by themselves. Non-synonymous SNPs that trigger an acid that is amino in the protein item are of major interest, because amino acid substitutions presently account fully for about 50 % of this understood gene lesions accountable for individual inherited illness ( 1). SIFT ( S orting we ntolerant F rom T olerant) utilizes series homology to anticipate whether an acid that is amino will impact protein function and therefore, possibly change phenotype ( 2, 3).